.. Tris(hydroxymethyl)aminomethane abbreviated as Tris or TRIŽMA is frequently used to make buffers in biochemistry laboratories. Tris has a Molecular weight 121.1 g/mol and its solution in water is basic with pH 10.5. Describe in details how to prepare 1L of 0.5 M Tris buffer solution at pH 7.5. . If 2L of 0.05 M Tris-HCI buffer solution pH = 7.5 are required for an experiment on column chromatography. Describe in details how to prepare this buffer solution by diluting the buffer prepared in question # 1. %3D

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Chapter16: Acid-base Equilibria
Section: Chapter Questions
Problem 16.24QP: You have 0.10-mol samples of three acids identified simply as HX, HY, and HZ. For each acid, you...
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1. Tris(hydroxymethyl)aminomethane abbreviated as Tris or
TRIZMA is frequently used to make buffers in biochemistry
laboratories. Tris has a Molecular weight 121.1 g/mol and
its solution in water is basic with pH = 10.5. Describe in
details how to prepare 1L of 0.5 M Tris buffer solution at
pH 7.5.
2. If 2L of 0.05 M Tris-HCI buffer solution pH = 7.5 are
required for an experiment on column chromatography.
Describe in details how to prepare this buffer solution by
diluting the buffer prepared in question # 1.
Transcribed Image Text:1. Tris(hydroxymethyl)aminomethane abbreviated as Tris or TRIZMA is frequently used to make buffers in biochemistry laboratories. Tris has a Molecular weight 121.1 g/mol and its solution in water is basic with pH = 10.5. Describe in details how to prepare 1L of 0.5 M Tris buffer solution at pH 7.5. 2. If 2L of 0.05 M Tris-HCI buffer solution pH = 7.5 are required for an experiment on column chromatography. Describe in details how to prepare this buffer solution by diluting the buffer prepared in question # 1.
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