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Apr 25, 2024

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1 Experiment 7 – Beer’s Law: Post-Lab (60 Total Pts) Post-Lab Results & Data Analysis Base Instructions for All Post-Labs Own Work Policy: All content submitted in this Post-Lab, with the exception of raw data from the In-Lab, must be your own work. Per the syllabus, you are not allowed to work on any post-lab questions or data workup with other students. This includes your lab partner. Any violation of this rule will be reported to the CHEM 101L instructor, and major or repeated offenses may result in referral to the Honor Court. Note that this requirement does not mean that you cannot receive assistance from TAs during office hours, and we encourage you to use this resource if you are having trouble. Figure Captions: For each figure caption, please provide just enough information about the graph/plot so that the reader understands what is being displayed. Usually, the first sentence gives an overview, and the following sentences give specifics. Example: “Reaction yield as a function of added catalyst with reaction yield determined by absorption measurements. Reactions were held at a constant temperature of 22±2 °C. Concentrations of all other reactants were kept constant at the values shown in Table 9. Each data point is the average of three trials.” Table Titles: Each table should include a one phrase title that describes the contents of the table. The title should be understandable without needing to read what’s in the lab manual. Example: “Rate constants for substitution reaction A as a function of temperature.” Units for Table Entries: For each entry in a table, units should be included in the column header unless the quantity is unitless. Units are generally not included in the body of the table itself to avoid clutter. Submission to Gradescope: When uploading to Gradescope, you will need to indicate which page each table, figure, or question is located on. Failure to do so will result in a point deduction for formatting errors. To do this, select the item from the Question Outline on the left side of the screen. Then, on the right side of the screen, select the page from your submission that contains this item.
CHEM 101L - Experiment 7 Post-Lab Fall 2023 2 A. Beer’s Law Calibration Plot ( 6 points ) Provide your Beer’s law calibration plot below. You may use either your Excel or your Vernier Graphical Analysis Plot, but you must include axes labels, a key/legend, and a linear trendline. You should have only one trendline for all 15 data points. Figure 1. Beer’s Law calibration plot expressing absorbance at 507 nm versus concentration (mol/L). Each data point is an average of 3 trials. Beer’s Law Calibration Plot: Absorbance vs Concentration
CHEM 101L - Experiment 7 Post-Lab Fall 2023 3 B. Calculating Concentrations Using the Beer’s Law Calibration Plot ( 10 points ) This section of the Post-lab is intended to guide you through the application of Beer’s law to determine concentration from an absorbance measurement. Follow along with the instructions and show your work in the answer box below. You may choose to upload an image of your work done by hand, but it must be legible. a) Re-arrange the equation for Beer’s law ( A = ε · · c ) so that concentration, c , is the only value on the right side of the equation. Then flip the sides so that c is on the left (i.e c = … ) b) Record the value of the path length, , of the cuvettes used in this experiment. c) Record the value of molar absorptivity, ε . The value of ε is the slope of the linear fit of the Beer’s law calibration plot above. d) Rewrite the re-arranged equation from part a) by substituting the values of ε and from parts b) and c) , respectively. e) Select one of the trials in which you measured the absorbance of your Gatorade sample. Starting from the equation you found in part d) , find the concentration, c , of this sample from its absorbance, A . Please show your work, including units and the appropriate number of significant figures. a. c=A/ ε* ℓ b. ℓ=1.000 cm c. ε=20160 ࠵? !࠵? ࠵?࠵? !࠵? d. c=.023 /20160 ࠵? e. concentration is in M, not units in image
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