BIO 110 Diffusion and Osmosis Lab Part III (Continuation of last week's lab Diffusion and Osmosis Pa

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Mars Hill College *

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Chemistry

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Apr 25, 2024

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Diffusion and Osmosis Part III: Lab Exercise #5 (Continuation of Diffusion and Osmosis Part II) Day 3: Exercise #5: Concentration gradients and the rate of osmosis In this exercise, you will design an experiment to test the following hypothesis: “The rate of osmosis increases as the difference in solute concentration across a selectively permeable membrane increases”. Before watching the experimental setup video or observing the results on page 3, use the space below to design an experiment to test the hypothesis provided above. Your experimental protocol must include enough detail that another student can use it to repeat the experiment. The materials available for the experiment are listed below. Materials available 0.2 M sucrose solution 0.4 M sucrose solution 0.6 M sucrose solution 0.8 M sucrose solution 1.0M sucrose solution water Dialysis tubing and clamps Electronic balance Glass dishes Graduated cylinders Funnels Experimental Protocol 1. Set up all the materials a. 1 dialysis bag with a pair of clamps, glass dish, graduated cylinder, and funnel for each sucrose solution & water. 2. Secure each dialysis bag on one side then begin to add the sucrose solution/water using the funnel and then secure the other side making sure the
solution will not leak out. a. Repeat this for all dialysis bags and solutions. 3. After each solution or water has been secured in a dialysis bag begin to weigh the solutions on the electronic balance. a. Be sure to record the data in the table. 4. Wait 60 mins and then begin to re-weigh each dialysis bag with solution and record the data. Now the you have generated an experimental protocol to test the hypothesis, watch this video to review the experimental set-up. Data on the mass of the dialysis bags at the start (0 min) and end (60 min) of the experiment has been collected and is presented in Table 1 below. Data Collection Table 1. Solution inside dialysis bag Mass (g) of dialysis bag at start (0 min) Mass (g) of dialysis bag at end (60 min) Water 15.25 15.49 0.2 M sucrose 15.85 16.43 0.4 M sucrose 15.6 16.66 0.6 M sucrose 15.2 16.4 0.8 M sucrose 15.64 17.41 1.0 M sucrose 15.65 17.73
% change in mass over 60 minutes Final mass - initial mass x 100 = % in mass Initial mass Graph Determine which type of graph will best present the experimental data. Construct the graph using google sheets and paste the graph into the space below.
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