BIOD171 Microbiology Lab Notebook

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CUNY Hunter College *

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Biology

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Feb 20, 2024

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docx

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Portage Learning BIO 171- Microbiology Lab Notebook Lab Notebook Bookmarks (click to navigate): Lab 1 Notebook Lab 2 Notebook Lab 3 Notebook Lab 4 Notebook Lab 5 Notebook Lab 6 Notebook Lab 7 Notebook Lab 8 Notebook Lab 9 Notebook
Lab 1 Notebook Back to Home Page Title: #1 Introduction to Medical Microbiology Objective: Cultivation of samples (growth conditions) - Equipment used Identification of samples (biochemical assays) - Tests available Evaluation of samples (microscopy) - Visualization of recognition of key characteristics Procedure: Cleaning: - Autoclave 125° C Growing - Fixed incubator 37° C - Shaker incubator 37° C Visualizing - Microscopy Storing - Refrigerator 4° C Lab safety:
Never eat or drink in the lab - contamination risk Use personal Protective Equipment (PPE) - Latex or thermal gloves (application dependent) - Eyewear protection - Lab coat Never leave the lab while wearing PPE - Public hallway - Bathroom - Cafeteria Notes: Additional helpful information placed here Results: A summary of the final outcome of the experiment should be here
Lab 2 Notebook Back to Home Page Title: Basics of Microscopy Objective: To become familiar with the basic components of a light microscope as well as how to load a sample for viewing. Procedure: 1. Review parts and components 2. Load sample slide into microscope 3. Select magnification 4. Make necessary adjustments to optimize sample visualization Notes: Parts of microscope: 1. Eye pieces (Ocular lens): Can be adjusted to fit the width of your eyes. Should be able to use both eyes but you should see a single circle within the viewfinder. 2. Arm/neck: When moving the microscope keep one hand on the neck and another underneath the base. 3. Objectives: Lenses that provide part of the magnification to the microscope. 4 different objectives with revolving nose. Shortest objective 4x magnification, others include 10x, 40x, and 100x. 4. Stage: flat surface where you place the sample. Includes a clamp holder and stage clips to hold the sample in place. 5. Focus knobs: Outside ring is called course adjustment used when you want to make large steps in changes of your focus. Inner knob is called fine focus used when you can see the object but need to focus more to see details. 6. Iris or Diaphragm: Controls how much light is let into the microscope and ultimately into the eyepiece.
7. Base: where the microscope sets. Always make sure the base is steady. 8. Glass slide: Located on the microscope stage. 9. Stage guide: There are two knobs. One knob controls vertical axis to move forward and backwards, another knob to move left and right. Visualization: - Types of objectives: Dry vs Oil - Intensity of light source: Too bright= saturation, Too dark= low visibility - Power of objective x Power of eyepiece= Total magnification: If a cell is 15 mm in diameter, using a 40x objective and 5x eyepiece the cell will appear to the eye 200 times larger or around 3000mm in diameter. Results: Magnification = object x eyepiece
Lab 3 Notebook Back to Home Page Title: Mounting Techniques Objective: Microscopic examination of bacterial samples through various staining techniques. Identify color and shape of given samples. Procedure: Dry Mount: 1. Clean slide (70% ethanol) 2. Circle area on slide for easy location of specimen (optional) 3. Apply organism to slide: If from culture, use sterile loop to spread onto slide. If from plate, use sterile loop to pick colonies and mix with a drop of distilled water. 4. Air dry at room temperature until all moisture has evaporated. Wet mount: 1. Clean slide (70% ethanol) 2. Circle area on slide with a wax/hydrophobic pen 3. Apply organism to slide: If from culture, use sterile loop to spread onto slide. If from plate, use sterile loop to pick colonies and mix with a drop of distilled water. 4. View under microscope: Wet mount is ideal for viewing the motility of an organism. Do not dry out. Gram Staining: 1. Clean slide (70% ethanol) 2. Apply organism to slide: Use sterile loop to spread ~ 1-3 drops onto slide. Spread into a thin film 3. Allow to air dry
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