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- What are the mechanisms of samples separation work in Thin layer Chromatography? Please shortly write at your own words. Answer should be to the point (5-6 lines maximum).a) What is the dilution factor if you add a 1ml aliquot of a specimen to 99ml of diluent? b)How to make 500ml of a 1:250 dilution?What is the underlying basis for the use of oil when using an oil immersion objective?
- Give a vivid outline of the Standard operating procedure of neutralization test.make the test principle, materials required for the analysis, sample preparation, test procedure, indication of a positive result, complications/interferences, for nitrous acid test.Mary Jo prepared a series of diluted samples by taking 1 mL of sample and adding it to 4 mLs of diluent. She then diluted the sample further by adding 1 mL of diluted sample to 19 mLs of diluent. What is the final dilution factor ?