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The Kjeldahl method was used to determine the nitrogen content of a protein. A 275 µL aliquot of a 47.0 mg/mL protein solution was digested in boiling sulfuric acid. The solution was made basic and the liberated NH3 was collected in 5.00 mL of 0.0512 M HCl. A volume of 7.00 mL of 0.0174 M NaOH was required to react with the excess HCl. Calculate the weight percent of nitrogen in the protein. weight percent: wt% N

The Kjeldahl method was used to determine the nitrogen content of a protein. A 275 µL aliquot of a 47.0 mg/mL protein solution was digested in boiling sulfuric acid. The solution was made basic and the liberated NH3 was collected in 5.00 mL of 0.0512 M HCl. A volume of 7.00 mL of 0.0174 M NaOH was required to react with the excess HCl. Calculate the weight percent of nitrogen in the protein. weight percent: wt% N

Chemistry: Principles and Reactions
Chemistry: Principles and Reactions
8th Edition
ISBN:9781305079373
Author:William L. Masterton, Cecile N. Hurley
Publisher:William L. Masterton, Cecile N. Hurley
Chapter4: Reactions In Aqueous Solution
Section: Chapter Questions
Problem 58QAP: The iron content of hemoglobin is determined by destroying the hemoglobin molecule and producing...
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The Kjeldahl method was used to determine the nitrogen content of a protein. A 275 µL aliquot of a 47.0 mg/mL protein solution was digested in boiling sulfuric acid. The solution was made basic and the liberated NH, was collected in 5.00 mL of 0.0512 M HCl. A volume of 7.00 mL of 0.0174 M NaOH was required to react with the excess HCl. Calculate the weight percent of nitrogen in the protein. weight percent: wt% N
Transcribed Image Text:The Kjeldahl method was used to determine the nitrogen content of a protein. A 275 µL aliquot of a 47.0 mg/mL protein solution was digested in boiling sulfuric acid. The solution was made basic and the liberated NH, was collected in 5.00 mL of 0.0512 M HCl. A volume of 7.00 mL of 0.0174 M NaOH was required to react with the excess HCl. Calculate the weight percent of nitrogen in the protein. weight percent: wt% N
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