The following is a Mannitol Salt Agar plate. What does the yellow color on the right side of this plate indicate? www. TAN ASM's MicrobeLibrary Ⓒ Reynolds tolerance of the salt mannitol metabolism tolerance of the dyes and chemicals in the agar Olactose metabolism
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- O The following is a Mannitol Salt Agar plate. What does the growth on this plate indicate? www TAN ASM's MicrobeLibrary Reynolds lactose metabolism tolerance of the dyes and chemicals in the agar tolerance of the salt mannitol metabolismThe following is a picture of Eosin Methylene Blue Agar. What does the green shimmer on this plate indicate? ASM MicrobeLibrary.org © Cheeptham CON LASZAMSAL lactose metabolism Otolerance of the dyes and chemicals in the media tolerance of the salt mannitol metabolismAspergillus can be used to produce α-amylase (α-AM), which is an enzyme that hydrolyzes the glycosidic bonds in starch molecules into maltose (disaccharide). Pure α-AM has a specific activity of 56.00 U/mg. The α-AM is secreted into the culture media during production (after 5 days, production is complete). Following, the Aspergilluscells are separated by centrifugation and discarded. The culture supernatant (with α-AM) is filtered giving a clarified supernatant with a volume of 100.0 L, activity of 3.00 U/mL, and total protein concentration of 0.650 mg/mL. After purification, the product stream (7.50 L) contains α-AM (25.00 U/mL) and a total of 6.788 x 103 mg total protein. Calculate the following: Concentration of total protein (g/L) and total units of activity in the clarified supernatant and purification product stream. Specific activities (U/g) of the clarified supernatant and purification product stream. Consider significant figures in your answers (may want to use scientific…
- List the ingredients and recipe (in gram or milligram) to make one liter (1000 ml) of Trypticase Soy Agar, nutrient agar, and Columbia blood medium (use a tableDescribe how a Triple Sugar Iron agar tube would appear if the following situation was true. Please note that all items in the following list are occurring in the same tube of agar. 1. Positive for glucose fermentation 2. Negative for lactose and sucrose fermentation 3. Positive for H2S production 4. Negative for gas productionThe following is a picture of Eosin Methylene Blue Agar. What does the growth on this plate indicate? ASM MicrobeLibrary.org © Cheeptham 15146, Olactose metabolism tolerance of the dyes and chemicals in the media Otolerance of the salt Omannitol metabolism
- The plate below is a test of 7 different antibiotics against Enterobacter aerogenes. The center disc is Ciprofloxacin 15 micrograms) and the 24 hour extent of the Zone of Inhibition is the farther diameter. The one below it and slightly to the right is Chloramphenicol (30 micrograms) [see red arrow}. Give me the extent of the Zone of Inhibition in mm (yes, use a ruler and measure it at the size it comes up on your computer screen), then use the Standard Table and determine R, I, S for each. Antibiotic Zone of Inhibition (mm) R, I or S Drug target Ciprofloxacin Chloramphenicol Which of the 2 drugs is more selective and why?Phenol Red Test Questions: Suppose you innoculate a phenol red broth with a slow-growing fermenter. After 48 hours, you see slight turbidity but score it as (-/-). Is this result a false positive or a false negative? Is this a result of poor specificty or poor sensitivity of the test system?If the inoculated lactose broth cube exhibits acid production but without gas formation, will you consider this as a negative or positive result? Explain Why is the MPN test qualitative rather than quantitative? Why is the MPN test qualitative rather than quantitative? Why is MPN test qualitative rather than quantitative
- What nutrients do the following media components contain? Peptone Yeast extract Beef extract Potato infusion Agar Why should agar media be completely dissolved before they are dispensed in tubes and plates? What are the bases for pegging the temperature at 1210C for 15-30 minutes during moist heat sterilization and 1800C for two (2) hours using dry heat sterilization? 1.Can you sterilize culture media using dry heat sterilization? Why is that so? You will notice in the videos shown, the cotton plug is not used. What is role of cotton plug in media prep, sterilization and culture of microorganisms? Instead of using cotton plug, plastic screw-cap is used, can you substitute this for the former? Is it technically acceptable in microbiology?Please include sourceShort description of the process occurring at each step of the extraction in the miniprep process indicating the purpose/action of the reagents usedAll of the following agars are selective and/or differential, Except: Group of answer choices Mannitol salt agar MacConkey agar Eosin methylene blue agar phenylethyl alcohol agar Nutrient agar