Reaction rate data of an enzyme, E, and a substrate, S, with increasing inhibitor concentrations are listed in the table below (Cs and C₁ represents the concentrations of the substrate and the inhibitor respectively). Reaction Rates (umol.L¹.s"¹) [CS] (mmol/L) [CI]=0 [CI]=0.25 [CI]=0.50 mmol/L mmol/L mmol/L 0.10 0.79 0.57 0.45 0.15 1.11 0.84 0.66 0.20 1.45 1.06 0.86 0.30 2.00 1.52 1.22 (a) Calculate Vmax (mmol.m³.s¹). (b) Calculate KM (g.mol.m³). (c) Describe the type of this inhibition. (33 points)
Reaction rate data of an enzyme, E, and a substrate, S, with increasing inhibitor concentrations are listed in the table below (Cs and C₁ represents the concentrations of the substrate and the inhibitor respectively). Reaction Rates (umol.L¹.s"¹) [CS] (mmol/L) [CI]=0 [CI]=0.25 [CI]=0.50 mmol/L mmol/L mmol/L 0.10 0.79 0.57 0.45 0.15 1.11 0.84 0.66 0.20 1.45 1.06 0.86 0.30 2.00 1.52 1.22 (a) Calculate Vmax (mmol.m³.s¹). (b) Calculate KM (g.mol.m³). (c) Describe the type of this inhibition. (33 points)
Chemistry & Chemical Reactivity
10th Edition
ISBN:9781337399074
Author:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Publisher:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Chapter14: Chemical Kinetics: The Rates Of Chemical Reactions
Section: Chapter Questions
Problem 45PS
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