In some cases, the Km and Vmax can be estimated by inspecting the values of the initial velocity (V at increasing [S]. Use the data above to ESTIMATE the approximate Km and Vmax O Km = 1000 micromolar %3D = 0.5 millimolar/sec O Km = 1 micromolar Vmax = 0.5 millimolar/sec %3D O Km = 1000 micromolar Vmax = 0.25 millimolar/sec O Km = 200 micromolar
In some cases, the Km and Vmax can be estimated by inspecting the values of the initial velocity (V at increasing [S]. Use the data above to ESTIMATE the approximate Km and Vmax O Km = 1000 micromolar %3D = 0.5 millimolar/sec O Km = 1 micromolar Vmax = 0.5 millimolar/sec %3D O Km = 1000 micromolar Vmax = 0.25 millimolar/sec O Km = 200 micromolar
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
Transcribed Image Text:You have measured the following data for enzyme X.
Substrate
Vo
concentration (micromolar
(mM)
Isec)
0.05
10
0.1
60
0.5
175
1
250
350
490
10
492
20
494
50
499
100
498
200
498
![In some cases, the Km and Vmax can be estimated by inspecting the values of the initial velocity (Vo)
at increasing [S]. Use the data above to ESTIMATE the approximate Km and Vmax
O Km = 1000 micromolar
%3D
Vmax = 0.5 millimolar/sec
O Km = 1 micromolar
Vmax = 0.5 millimolar/sec
%3!
Km = 1000 micromolar
%3D
Vmax
= 0.25 millimolar/sec
O Km = 200 micromolar
Vmax = 500 millimolar/sec
%3D](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fc958f3a6-a2a0-43f6-8da2-984adc6ab0d2%2F48d3bf06-380f-470b-9ea2-5ecd5d9b6dcd%2F3q21vx9_processed.jpeg&w=3840&q=75)
Transcribed Image Text:In some cases, the Km and Vmax can be estimated by inspecting the values of the initial velocity (Vo)
at increasing [S]. Use the data above to ESTIMATE the approximate Km and Vmax
O Km = 1000 micromolar
%3D
Vmax = 0.5 millimolar/sec
O Km = 1 micromolar
Vmax = 0.5 millimolar/sec
%3!
Km = 1000 micromolar
%3D
Vmax
= 0.25 millimolar/sec
O Km = 200 micromolar
Vmax = 500 millimolar/sec
%3D
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