Q: Both crystal violet and safranin are basic stains and may be used to do simple stains on Gram…
A: Gram staining is the common procedure followed to distinguish the bacteria based on their cell wall.…
Q: Compare and contrast how dark-field, phase-contrast, differential interference contrast,…
A: The technique by which the smaller or minute particles are magnified for studying the features is…
Q: Explain why only gram-negative cells undergo decolorization during the gram staining procedure.
A: Gram staining is a method in which cells stain either pink (gram-negative) or purple (gram-positive)…
Q: What is the importance of Gram-staining?
A: Introduction : Gram Staining, Commonly Known As Gram's Method, Is A Staining Technique That Is Used…
Q: Define fluorescent staining.
A: The advent of microscopy started with the development of optical microscopy. Then the level of…
Q: You receive a bacterial culture in the lab and your job is to figure out information which will be…
A: Best option to stain a bacterial culture is Gram Stain Method. It is used to determine gram status…
Q: Enumerate the advantages derived from staining bacteria.
A: BASIC INFORMATION BACTERIA It is one of the type of biological cell. They belong to prokaryotic…
Q: In Gram staining technique, Decolorizing agent step should take Select one: O a. 60 seconds O b. 30…
A: Usually, Acetone is used as a decolorizer but some prefer ethanol and acetone in a 1:1 ratio.…
Q: Please discuss why staining is important in visualizing microbes under the microscope. What…
A: Staining is an essential step in visualising microbes under the microscope. THE NECESSITY OF…
Q: Which of the following are true about a differential stain. There may be more than one answer!…
A: A microscopic organism made of one cell is referred to as a prokaryote. Prokaryotic organisms do…
Q: Bacillus subtilis (Gram-positive) and Pseudomonas aeroginosa (Gram-negative) bacteria are heat-fixed…
A: Bacteria have cell walls made up of polysaccharides that give them strength.Bacterial cell walls…
Q: In your own words, explain how gram staining is done and how it works.
A: Gram staining is a popular method for distinguishing between two large groups of bacteria based on…
Q: Give the principle of acid fast staining and gram staining.
A: Microorganisms are extremely small in size and hence cannot be visualized directly. Specialized…
Q: Describe how simple staining and fluorescence staining are similar and how they are different. What…
A: Microscopy is a technical field for the use of microscopes to visualize objects and locations of…
Q: When doing a gram stain in microbiology, one step can be eliminated and still allow distinction…
A: Please follow step 2 for detailed explanation.
Q: Outline the steps in gram staining
A: The common microbial technique that is used to differentiate the two large groups of bacteria…
Q: Is acid fast staining differential stain , if yes, what are the 2 types of stain used
A: Differential Staining is defined as a staining process which utilizes more than one chemical stain.…
Q: list 4 advantages and 4 limitations of the Gram staining process
A: Gram staining process is the procedure which is uses differential gram stain which has two dyes to…
Q: Explain the difference in the working principles of Dark-field and Light-field microscopes using pKk…
A: Microscope: It is an optical instrument that uses a lens or a combination of lenses to produce…
Q: Types of Differential Staining techniques. 1. Gram stain 2. Acid-fast stain Please…
A: Staining is used in microscopy to highlight the structures to be viewed under microscope. It is of…
Q: staining
A: Staining- It is used to enhance contrast in samples, generally at the microscopic level. In other…
Q: 1.)What is the purpose of a counterstain?
A: BASIC INFORMATION ENZYMES They are the catalyst. They help in accelerating the chemical reaction.…
Q: Briefly discuss the difference between simple and differential staining? Which is better suited for…
A: Staining is a laboratory technique in which various coloured dyes are used to highlight the specimen…
Q: If you forgot to apply the safranin counterstain while performing a Gram stain, which outcome would…
A: Gram staining is a staining technique used to differentiate two large groups of bacteria based on…
Q: List the steps in a Gram stain.
A: Introduction: The principle of Gram's stain depends upon the Gram's reaction. The Gram's reaction…
Q: Based from the results obtained from Gram staining, explain why bacterial cells retained or changed…
A: Staining is a technique that is used to enhance the contrast of the microscopic image through the…
Q: Explain the principle of Gram staining. (You do not need to describe the procedure step by step).
A: The danish Bacteriologist Christian gram discovered this differential technique in order to…
Q: 2. Draw and label a microscopic presentation of: Positive Stain (indicate stain used) Negative stain…
A: Gram staining is a technique used to differentiate between two groups of bacteria namely…
Q: write the protocol for mann's methyl blue-eosin staining, including their reagents, materials,…
A: Reagents: Alkaline alcohol reagent –100ml of absolute Ethanol or Methanol containing 0.2ml 0.1N…
Q: - After you are finished, list each step for the Gram staining procedure. - Which of the four steps…
A: NOTE: AS PER OUR GUIDELINES WE NEED TO ONLY ATTEMPT FIRST QUESTION AND ITS PARTS , PLEASE RESUBMIT…
Q: Explain the mechanism of Gram staining.
A: Gram staining is the technique used to classify the bacterial species into broad groups based on the…
Q: Explain the relationship between bacterial cell wall with gram-positive and gram-negative in the…
A: Gram staining is the standard laboratory practice for distinguishing between two broad species of…
Q: Select all of the following that represent examples of positive stains. Check All That Apply…
A: Staining is the common laboratory procedure which can be specific and general according to the use.…
Q: Write out the steps for this specfic stain. List them in the correct order
A: Organisms can be tentatively recognized via developing them on media and searching at morphology or…
Q: Define the term gram staining.
A: Bacteria are a diverse group of microorganisms with a wide range of shapes, sizes, structures, and…
Q: This part of the staining process helps the primary stain to remain in the cell during…
A: Staining of biological samples is a very useful process for detailed observation and measurement of…
Q: Write the protocol for lactophenol blue stain method indicating; materials, reagents, Principle,…
A: Fungi consist of a broad class of organisms from single yeast to multicellular organisms like…
Q: the steps in doing gram staining using a flow chart.
A: Gram-staining is a process based on the ability of bacteria to retain crystal violet dye. The…
Q: Evaluate the following preparations in terms of providing informationon microbial size, shape,…
A: Staining is an important step in observing the microbes under a microscope. they allow to the…
Q: Explain in detail simple and differential staining and also improving and adjusting contrast in…
A: Staining is a chemical method which is used to colour the organism or the cell so that it is viewed…
Q: What type of stain is the Gram stain and what does it rely on for meaningful results
A:
Q: What color are Gram negative cells after decolorizing with ethanol? What is the best age for your…
A: Since you have posted a question with multiple sub-parts, we will solve the first three sub-parts…
Q: Describe the procedure for performing a Gram stain. What is the procedure? How does each component…
A: * Gram staining is used to test that whether f bacteria is positive or negative and also be used to…
Q: Describe the procedure for performing a Gram stain. ▪ What is the procedure? ▪ How does each…
A: Bacteria are ubiquitous microscopic , one celled organism which possess variety of shape ,…
Q: Gram-staining
A: Reagents using in Gram-staining are : Crystal violet Iodine Alcohol/Acetone Safranin
Q: Of the four major types of microscopes, give an example of a scenario in which each would be the…
A: Electron microscopy is a tool that allows us to see tiny images of objects that would otherwise be…
Q: Briefly describe the uses of Special stains.
A: Routine stain: It refers to the hematoxylin and eosin (H&E) staining that is used routinely to…
Q: Explain the importance of steam during the acid-fast staining procedure. Discuss the advantages of…
A: Some microbes and their structure are very difficult to be observed under the microscope . So , for…
Q: In preparing bacterial smear, why do we need to pass the slide over the flame?
A: since this is a multi-part question, so I am answering only 1st part. please upload other questions…
Q: For each of the following stains, list each of the chemicals used (in order), briefly describe what…
A: In Microbiology staining is needed in order to properly visualize the internal membranes of the…
Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of simple
1. Simple Staining
Step by step
Solved in 2 steps with 2 images
- Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of simple staining and gram staining. 2. Gram StainingExplain the importance of steam during the acid-fast staining procedure. Discuss the advantages of differential staining procedures over the simple staining techniques. List and briefly describe the functions/purpose of each of the different stains used in acid fast staining.name 5 things about method staining.
- Describe how simple staining and fluorescence staining are similar and how they are different. What are the advantages of each?Give the principle of Grams stain, Ziehl-Neelsen, and Kinyoun Staining methodsBriefly discuss the difference between simple and differential staining? Which is better suited for common clinical microbiology laboratory applications?
- Select all that apply to a direct simple stain: 1. the dye is acidic or negatively charged and stains the background 2. uses multiple dyes in the procedure 3. cells will never be distorted or shrunken 4. uses only 1 dye in the procedure 5. does involve a washing step 6. can be used to determine cell morphology, size, and arrangement. 7. does involve fixing 8. the dye is basic or positively charged and stains the bacteria 9. cells may be distorted or shrunken 10. does not involve a washing step 11. does not involve fixingBriefly explain the difference between Wright Staining and H&E Staining (why use one over the other)Choose the 4 most correct steps from the list below for a simple positive stain and organize them into the most correct order. Briefly explain the purpose of each step you chose. • Heat fix • Dip slide in acid alcohol • Wash slide with ethanol • Apply Congo Red to the slide • Apply Crystal Violet to the slide • Make a smear • Wash slide with water
- Describe the procedure for performing a Gram stain. ▪ What is the procedure? ▪ How does each component work? (ie. What does the mordant do?)a: What are the components of a Romanowsky stain? b: What is the optimum pH for staining with Giemsa. c: Name two conditions which will affect the quality of your staining. d: What is a panoptic stain. Give one example. e: Name two blood parasites that can be demonstrated using the Giemsa stain.Give the principle of acid fast staining and gram staining.