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I am not sure how to go about illustrating and answering this question: Use a diagram to illustrate how 10^-4, 10^-6 and 10^-8 dilutions in water of the soil suspension in a final volume of 10 mL will be prepared. There are only three tubes available.

I am not sure how to go about illustrating and answering this question: Use a diagram to illustrate how 10^-4, 10^-6 and 10^-8 dilutions in water of the soil suspension in a final volume of 10 mL will be prepared. There are only three tubes available.

Curren'S Math For Meds: Dosages & Sol
Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter20: Iv Medication And Titration Calculations
Section: Chapter Questions
Problem 1.3P
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I am not sure how to go about illustrating and answering this question: Use a diagram to illustrate how 10^-4, 10^-6 and 10^-8 dilutions in water of the soil suspension in a final volume of 10 mL will be prepared. There are only three tubes available.
Exercise 1.3 Use a diagram to illustrate how 10-4, 10-6 and 10-8 dilutions in water of the soil suspension in a final volume of 10 mL will be prepared. Note you only have three tubes available.
Transcribed Image Text:Exercise 1.3 Use a diagram to illustrate how 10-4, 10-6 and 10-8 dilutions in water of the soil suspension in a final volume of 10 mL will be prepared. Note you only have three tubes available.
EXERCISE 1.3: MPN OF A SOIL SAMPLE (Groups of 2) Materials lg of soil Flask with 100 mL of sterile water 100 mL sterile water 18 X 5 mL TSB Sterile tubes Method 1. Transfer 1g of soil to the flask containing 100 mL of sterile water. (This represents a 10-² dilution) Shake on shaking platform for 10 minutes. 2. Allow the soil to settle for 10 minutes. 3. Prepare 104, 10-6 and 10-8 dilutions in water of the soil suspension in a final volume of 10 mL. 4. Perform an MPN count as indicated below in six sets of three tubes containing 5 ml of TSB. 5. Incubate at 37°C for 24 hours after which they will be stored at 4°C until your next lab period. Sample 10-4 10-4 10-6 10-6 10-8 10-8 Volume of inoculum 1.0 mL 0.1 mL 1.0 mL 0.1 mL 1.0 mL 0.1 mL Vol. of medium 3 tubes 5 mL 5 mL 5 mL 5 mL 5 mL 5 mL
Transcribed Image Text:EXERCISE 1.3: MPN OF A SOIL SAMPLE (Groups of 2) Materials lg of soil Flask with 100 mL of sterile water 100 mL sterile water 18 X 5 mL TSB Sterile tubes Method 1. Transfer 1g of soil to the flask containing 100 mL of sterile water. (This represents a 10-² dilution) Shake on shaking platform for 10 minutes. 2. Allow the soil to settle for 10 minutes. 3. Prepare 104, 10-6 and 10-8 dilutions in water of the soil suspension in a final volume of 10 mL. 4. Perform an MPN count as indicated below in six sets of three tubes containing 5 ml of TSB. 5. Incubate at 37°C for 24 hours after which they will be stored at 4°C until your next lab period. Sample 10-4 10-4 10-6 10-6 10-8 10-8 Volume of inoculum 1.0 mL 0.1 mL 1.0 mL 0.1 mL 1.0 mL 0.1 mL Vol. of medium 3 tubes 5 mL 5 mL 5 mL 5 mL 5 mL 5 mL
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