GFP GROWTH -PGLO on LB +PGLO on LB -PGLO on LB/amp +PGLO on LB/amp +PGLO on LB/amp/ara

Q: student needed to transfer bacteria from a broth culture to an agar plate. Below is the step-by-step…

A: 5. The loop was flamed

Q: Do you think standard plate counts are very accurate? Why or why not? When doing serial dilutions,…

A: You have asked multiple questions. I will answer the first 3 subpart question, as allowed by…

Q: What is the purpose of flaming an inoculating loop?  How will you know when you have flamed the loop…

A: Hi, since you have posted multiple questions, we will be providing you with answers for the first…

Q: A culture of E. coli has a concentration of 5 x 108 cells/mL. How many times do you have to dilute…

A: Given: 1. Number of colonies: 2502. No. of bacteria in culture: 5 X 108 3. Volume plated: 0.1 ml 4.…

Q: Why are the results of plate counts expressed as the number of colony-forming units, rather than the…

A: Microbes are small living organisms  which can not be seen through naked eyes. The study of these…

Q: After proper incubation, you obtain your Mannitol Salt agar (MSA) plate and your MacConkey (MAC)…

A: MacConkey Agar is a selective and differential medium while MSA is a selective media and both are…

Q: You do a serial dilution on an actively growing bacterial culture broth. Doing a plate count of your…

A: A serial dilution is the stepwise dilution of a substance in solution.  It is done to find out the…

Q: You are culturing bacteria using a petri dish, the bacteria grow very well on this plate you have.…

A: Number of bacteria per ml of a sample is represented by colony forming units(CFU) per ml.CFU refers…

Q: What is the magnification of each of the following objectives? scanning:   low power:   high…

A: Microscope can produce the larger images of an object in contrast to its original size and this…

Q: Per the USDA, whole, unpasteurized fresh eggs can contain no more than 50,000 CFU/mL bacteria in a…

A: The serial dilution is a process that is routinely used in microbiology laboratories for the…

Q: equal volume of cell suspension + equal volume of Trypan Blue). Take this into account when…

A: The dilution factor is commonly represented by the magnitude relationship between the concentration…

Q: Students in a microbiology lab are provided with a culture of E. coli, and don't know how long it's…

A: Bacterial growth is increase in the quantity of cellular constituents and number .It depends upon…

Q: What is the best descriptive match for the following culture media? A. Specific biological…

A: Question no 1 Synthetic media: In this type of medium all the elements/chemicals used are well…

Q: You are given an unknown bacterial culture and you want to determine if it is resistant to…

A: Antibiotics are chemicals that fight bacterial infections in humans and animals. They work by…

Q: You begin with an overnight culture of approximately 108 organisms/ml and need a concentration of…

A: In an Overnight culture of the bacteria, their growth factor depends upon the type of bacteria we…

Q: In a Kligler Iron Agar/Triple Sugar Iron test you end up with a result with a yellow butt and red…

A: When a non-lactose fermenting bacterium hydrolyses the agar slant in the Kliger Iron Agar/ Triple…

Q: Can we use human blood for the preparation of blood agar? yes or No and explain why in full detail.

A: Blood Agar is an enhanced media with several nutrients that is commonly used as a base media for the…

Q: The lag phase does NOT occur if an exponentially growing culture is transferred into a nutrient rich…

A: Microbial growth is said to occur when there is an increase in the number of cells rather than an…

Q: You aseptically transfer 1 ml of your optional liquid culture into 99 ml of sterile water. What is…

A: The following are needed for calculating the dilution factor  1) Original volume of  solution we…

Q: You have a cell count of 6.7 x 10° cells / ml in a total volume of 4.5ml. You have resuspended your…

A: The concentration of bacteria depends on many factors like inoculum concentration, incubation…

Q: n this step, wash the cells with ice-cold PBS. Drain the PBS, then add ice-cold lysis buffer.…

A: The question you have asked lacks some background information about what you are trying to do, But…

Q: where 5 nutrient agar pates per female student and 3 agar slants per males student are needed to…

A: It is given that 5 nutrient agar plate prepared by per female so Seven female will prepare=7*5=35…

Q: 1. (2.5 pts) A pure bacterial culture of unknown concentration was diluted to determine the…

A: Given, each time 100 ul is added into 400 ul of diluent solution. Also,100 ul is added into culture…

Q: Remove the agar plate from its lid with the left hand. While holding the plate, inoculate the agar…

A: Introduction -- Microbes are around and every where in environment. Sterile agar plates which…

Q: Complete each sentence to demonstrate your understanding of the correct procedure for inoculating a…

A: Petri plates are the shallow cylindrical glass dishes that are used in the labs to culture the…

Q: After incubating for 24-48 hours, you retrieve your 4 agar plates for the osmotic pressure exercise…

A: Introduction The force with which the water enters the semipermeable cell membrane of an organism is…

Q: Show the calculations required to make up 250mlof a stock solution of this chemical that will then…

A: Introduction :- Dilution is the process through which we can make a solution less concentrated by…

Q: A patient sample will be analyzed. You are responsible to quantify the bacteria number. The dilution…

A: A sample of your blood, urine, skin, or any part of your body will be taken during a bacteria…

Q: A sample of well water is tested for its bacterial content in a plate count assay. A one-milliliter…

A: Given that, after incubation, the number of formed colonies are 83. One ml of fourth successive…

Q: Would you use an inoculating loop to transfer bacteria to an agar deep tube?

A: Agar deep tube is a glass tube which is filled with solid medium. The medium is solid due to…

Q: You perform a serial dilution of a water sample and the 10-7 tube when 0.1 ml was plated onto the…

A: Estimation of the number of viable cells is important in microbiology protocols. It is estimated…

Q: Citrate Test |Cetrimide Agar

A: Biochemical measures are used to differentiate bacterial organisms based on their biochemical…

Q: Gram-positive (+) bacteria with a thin peptidoglycan layer traps the crystal violet stain. Select…

A: Gram staining is also known as Gram's method is a staining method for identifying and separating…

Q: You do two 1:5 serial dilutions on an actively growing bacterial culture broth. Doing a plate count…

A: CFU or colony forming unit is a measurement or a unit that is used to estimate the concentration of…

Q: The picture shows bacteria (Escherichia coli) under a microscope Do you bacterial growth or no (…

A: * Escherichia coli is a rod shaped gram negative bacteria present in human intestine. * E coli…

Q: In the preparation of a pure culture media, the petri plates are inverted when placed in an…

A: In microbiology, culture media is considered the growth medium that possesses the specific mixtures…

Q: Agar was not completely set prior to inoculating with the organism. Effect: Rationale:

A: Bacterial cultivation requires specific types of medium that can provide some of the basic…

Q: Below is an MSA plate inoculated with bacteria and grown at 37*C for 2 days. Be pecific and thorough…

A: MSA plates inoculated with bacteria shows yellow color.

Q: Technique used to inoculate plate media using inoculating loop. Technique used to inoculate agar…

A: # Here I am giving solution of first two questions . Please repost your third question and better to…

Q: Which microbiology test measures the use of LACTOSE? What color is positive in each?

A: Lactose is a naturally occurring sugar present in dairy products such as butter and desserts.…

Q: Correctly identify the steps and outcomes in the process of screening for a nutritional mutant of a…

A: Culture medium or nutrient medium is a  place, mainly in petridis where microorganism utilise there…

Q: A pure bacterial culture of unknown concentration was diluted to determine the concentration of…

A: Viable plate count = 30 - 300 colonies

Q: For his experiment, Brian needs to prepare 30 plates (use maximum volume), 15 slants (small test…

A: Introduction Lysogeny Broth (LB) Is A Nutrient-dense Media Predominantly Used For Bacterial Growth.…

Q: You are tasked with quantifying bacteria in your soil sample. You resuspend 1.0g of soil in 10 ml 1x…

A: Given data:Number of colonies  = 175Volume plated = 150 uLTube dilution = 10^-4 or Dilution factor =…

Q: Transfer of Culture from Agar Plate to Agar Slant

A: The bacterial colonies of interest, after they are isolated from a particular source, need to be…

Q: bacterial suspension with a population of 6.3 x 107cells/mL was diluted as follows: 4 mL was…

A: By using the Aseptic technique, The initial dilution is made 4mL into 16mL So, firstly dilution…

Q: When testing the efficacy of an antibiotic against bacteria on an agar plate, it is important to…

A: The disk diffusion method, also known as the Kirby-Bauer test, is used to test the effectiveness of…

Q: Below is a diagram of the serial dilution of a culture with 1 x 10° cells. Fill out the missing…

A: Colony-forming unit / mL= Number of colonies * Dilution factor * 1/volume plated in mL CFU/mL = 1 *…

Q: Which of the following antibiotics exhibit the greatest antibacterial efficacy in the Kirby-Bauer…

A: Bacteria are prokaryotes belonging to the kingdom Monera. The bacteria can be both beneficial and…

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.