Explain the mechanism leading to the given reactions in the inoculated biochemical media. 1. Blackening of the medium in Triple Sugar Iron Test slant
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Explain the mechanism leading to the given reactions in the inoculated biochemical media.
1. Blackening of the medium in Triple Sugar Iron Test slant
2. Development of pink to red color in nitrate broth in Nitrate Reduction Test
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- 1. What are the two ways that nitrate can be reduced by the bacteria. Explain briefly. 2. What organisms are being differentiated in the butyrate disc test. Explain its reaction to the test.A 1.5-year-old child developed vomiting, diarrhea, and fever. Stool sample were inoculated into the Endo media. After 18 hours on the surface of the medium grew medium-sized, round, slightly convex red colonies with a metallic luster. The doctor suspected Escherichia coli. 1. Name the composition of the Endo agar media. 2. Describe the properties of bacterial colonies on the Endo media. 3. What purpose differential diagnostic Endo media used for?1. Give the importance and purpose of the Cetrimide Test. 2. being performed - Dry Filter Paper Method, Wet Filter Paper Method and Swab Method. 3. In the Oxidative-Fermentative Test, differentiate non-saccharolytic from oxidative from fermentative results.
- The following methods used for sterilizing an antibiotic solution. i. Dry heat sterilization ii. Microfiltration iii. Autoclaving iv. Desiccation Select one: O a. i and ii O b. i and iii O c. ii and iv O d. ii onlyWhich of the following is FALSE about the Nitrate Reductaion Test, procedure and interpretation? Question 2 options: A Nitrate medium contains 0.1% potassium nitrate as a substrate B Reagent A and B are used to determine the presence of nitrite C When zinc is added, it will react with any nitrate and turn red D A tube with red after zinc is a positive test for nitrate reduction E A tube that turns red after reagent A and B is a positive testWhich type(s) of medium would be used in each scenario?a. isolating the growth of Streptococcus pyogenes from a patient’s throat swabb. isolating a pathogen from a patient’s clean-catch urine samplec. isolating enteric bacteria such as Escherichia coli from a sample oforganically grown spinach d. maintaining a patient’s nasal swab specimen for further analysis andidentifi cation of possible respiratory syncytial virus (RSV) infection
- From figure shows triplesugar iron agar. What biochemical characteristics does this figureillustrate? How could this medium be used to begin the identificationof the isolated bacteria in this chapter’s Case Study?1. Give the importance and purpose of the Cetrimide Test. 2. Discuss briefly how each of the different oxidase method is being performed - Dry Filter Paper Method, Wet Filter Paper Method and Swab Method. 3. In the Oxidative-Fermentative Test, differentiate non-saccharolytic from oxidative from fermentative results.Which one of the following methods of antimicrobial susceptibility testing combines some of the properties of two other methods and involves adding a plastic strip to the agar? Select one: a. Agar macrodilution method b. Gradient diffusion method c. Broth microdilution method d. Disk diffusion method
- What are the advantages of the API 20E test compared to regular biochemical tubemediawrite a detailed description of each of the following tests/media: a. TSI Agar Slant b. SIM Medium c. Urease Test d. The IMViC Tests e. The Catalase Test f. The Oxidase TestChoose one specific way from each of the two methods of inhibiting the growth of microbes below. Describe the method, explain the mechanism of action of killing the microorganisms and give practical applications of your chosen ways. 1. Physical Methods - Heat, cold, dessication, radiation, ultrasonic waves, filtration, gaseous atmosphere 2. Chemical Methods- disinfectants, antiseptics