Explain the correlation between the structure of the compounds (b-Carotene, chlorophyll ) and the polarity of the solvent (hexane,70/30 hexane/acetone) that separated the compounds on the column. (TLC plate experiment on spinach)
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Explain the correlation between the structure of the compounds (b-Carotene, chlorophyll ) and the polarity of the solvent (hexane,70/30 hexane/acetone) that separated the compounds on the column. (TLC plate experiment on spinach)
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- 2. Consider the following example GC Chromatogram from a publication about aggression among Mediterranean Acrobat Ants (C. scutellaris). This particular sample was generated by rinsing hydrocarbons from ant exoskeletons. From the chromatogram, what is the minimum number of unique compunds present in the sample? What data led you to that conclusion? (Don't overthink it) 2 8000000 7000000 6000000 5000000 4000000 3000000 2000000 1000000 Counts (Abundance) 5 19 6 7 0 23.00 24.00 10 | 11 13 17 20 14 15 16 12 teel | 22 19 21 ли 25.00 26.00 27.00 Time "Example of a Gas chromatography-Mass spectrography (GS-MS) chromatogram of hydrocarbon molecules in C. scutellaris" by Filippo Frizzi, et.al, in PLoS One is licensed under CC BY 4.0Q2) In the thin layer chromatography experiment, calculate the relative mobility for the two dyes if the distance traveled by the solvent (ethanol) was 3 cm, the distance traveled by the purple dye was 2.5 cm, and 1.5 cm for the red dye? Which dye is considered less soluble in ethanol?1,8-Cineole is a predominant terpene found in cranberries. The concentration of 1,8-cineole in cranberries was determined using gas chromatography. A 5.00 g sample of freeze-dried cranberry powder was added to 10.0 mL water to form a cranberry extract. The extract was filtered. A 1.00 mL aliquot was added to a 25 ml volumetric along with a known amount of internal standard (d-14 cymene) and diluted to volume with water. Standards were spiked with a fixed amount of d-14 cymene as internal standard. The following data was obtained: 1,8-Cineole (ug/mL); 1,8-Cineole (peak area); d-14 cymene (peak area) 10.0; 1260; 1880 15.0; 1865; 1850 20.0; 2520; 1855 50.0; 6280; 1865 Sample; 1976; 1840 What is the concentration of 1,8-cineole in ug per gram of cranberry? O 398 ug/g O 159 ug/g O 674 ug/g O 796 ug/g O 15.9 ug/g
- Both melting points and TLC rely on differences in non covalent forces. Explain the role of these non-covalent interactions in the final answer obtained (melting point and Rf value).Use a suitable model to explain how separation and identification of a mixture of organic compounds can be achieved with a thin layer chromatographic (TLC) technique.In normal-phase HPLC chromatography, the stationary phase is polar and the mobile phase is nonpolar. In the reversed phase we have just the opposite; the stationary phase is nonpolar and the mobile phase is polar. Describe molecules in which move faster or slower in each case.
- 4. a. What physical property of a compound predicts whether that compound travels slowly or rapidly through a Gas Chromatograph? b. Suppose you injected a sample of 25% compound A. (bp 80 °C), 5% compound B (bp. 40°C) and 70% Compound C (bp 60 °C) on a GC. inject Circle the Chromatogram that actually fits that scenario and cross the other one out. On the correct GC, label the peaks on the chromatogram with the compounds A, B, and C. inject011/19 CH3 HO HO CH2 HO H HO H HO HO. H OH HO" Но H. 02,00~ CH3 Но HN ZHN" CH3 explain your choices. (9) Which of the following molecules might also make a suitable resolving agent for ibuprofen? Brieflyef Correlate the structures of the standard food dyes to their relative position in the paper chromatogram. Refer to the structures shown below. H3C. *Na O O HO. O CH3 Allura Red AC (pigment in red dye) 01510 106 FO O Nat O Nat O Nat FO O Na+ 0=8=0 HO 0=S=0 Na Na+ Tartrazine (pigment in yellow dye) Brilliant Blue FCF (pigment in blue dye) What are the identities of the unknowns in paper chromatography of food dyes? How did you arrive at this conclusion? Differentiate normal phase chromatography from reverse phase chromatography. From which of the two governs the experiment you did in thin layer chromatography of plant pigments? How do you say so?
- Identify these statements with true or false true or false I. Chromatography utilizes partition coefficient theory true or false II. In reverse phase chromatography, the mobile phase is a polar solvent true or false I. Aspirin is an ester prodrug. true or false Aspirin is synthesized by means of an acid catalyzed reaction. true or false I. Grain alcohol boils at 78.3 C true or false Wood alcohol boils at 60 C true or false Diazo coupling involves a sigma and a pi bond in two nitrogen atoms true or false When methyl orange passes through from basic to acidic medium, the change in color is from yellow to intense red true or false Benzoic acid is a white crystalline substance that has a melting point of 122 C true or false Benzoic acid can be prepared by the alkaline reduction of toluene true or false Potassium permanganate is an oxidizing agent true or false Potassium permanganate should never be mixed with glycerin true or false Methyl…TLC relies on differences in non-covalent forces. Explain the role of these non-covalent interactions in determining the Rf value obtained.Upon screening with TLC (made of silica gel) chromatography using hexane/chloroform (3:1) as eluting solution, 3 spots were detected corresponding to a (A) polyphenol, a (B) tannin and an (C) alkaloid. Write the letters of your answers on the spots in the chromatogram shown at the right.