Exercise 4: Slide smear (know what is and how to do - from plate cultures and broth cultures)- scenario assessments Simple stain (know what is and how to do) - scenario assessments Differential stain (know what is and how to do)- scenario assessments Negative stain (what is and how to do) Heat-fixation (what is and why do we do it)
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- INSTRUCTION: Give the principle or significance of the following practices: 1. Flaming the mouth or lid of culture tubes or plates after opening and before closing them. 2. Flaming the inoculating needle or loop before and after inoculation. 3. Holding caps, lids, or cotton plugs rather than putting them on the table while inoculating.INSTRUCTION: Give the principle or significance of the following practices: NOTE: *please add references* 1. Flaming the mouth or lid of culture tubes or plates after opening and before closing them. 2. Flaming the inoculating needle or loop before and after inoculation. 3. Holding caps, lids, or cotton plugs rather than putting them on the table while inoculating.EXPERIMENT 2 Title: Aseptic technique Objectives: To apply the aseptic technique To observe the growth found on the petri dish. Material/apparatus: Gloves, incubator, soap for handwashing, tissues, nutrient agar, 70% alcohol Procedure: Wipe off the bench with 70% alcohol. Draw a line down the middle of the petri dish to divide the plate in half. Label each halves with A and B. Press your unwashed thumb onto the agar at column A. Apply proper handwashing technique. Put the same thumb after washing onto agar at the column B. Incubate the petri dish at 37°C for 24 hours or overnight. Observe and note down the colonies. Results:
- :Objectives on Agar plates and line Streaking method-13 Two students applied following are the results they obtained :Student A Student B Who PSucceeded PWho failed? WhyFor the juice samples in exercise 3-4, you will be using the pour plate technique. In this exercise, you will add juice to a petri dish and pour agar over the sample and mix. Why is the media (potato dextrose agar, PDA) kept in a water bath until you are ready to use it, and why must you work fairly quickly? (One response answers both questions). The media is already solid and will form chunks The media has a dye added to it that must be kept at 45°C until ready to use O PDA is a media that allows growth of many types of fungi, and the heat from the water bath keeps the fungal spores from growing. The media is kept liquid in the water bath with heat, removing the tube from the heat source will cause the PDA to solidifyTOPIC: KATO-THICK & KATO-KATZ TECHNIQUES 1. What are the advantages and disadvantages of Kato-Katz technique? Briefly explain each.
- TOPIC: KATO-THICK & KATO-KATZ TECHNIQUES What are the differences between Kato-Thick smear and Kato-Katz technique? Briefly explain each.Direction: Read and analyze the following laboratory experiment and answer the following question. PART 1: SURFACE AREA AND CELL SIZE Materials: Agar containing NaOH, and the pH-indicator dye phenolphthalein cured into cubes of various size, 3 plastic cups, HCl, metric ruler, paper towels. Methodology: 1. Safety: Wear goggles and nitrile gloves while completing this lab. 2. Obtain three different size blocks of pink or blue agar. Using a ruler, measure the length, width, and height of the three blocks given below. Cut the agar according to the given dimension. Small = 1 cm x 1 cm x 1 cm Medium = 2 cm x 2 cm x 2 cm • • Large = 1 cm x 1 cm x 6 cm 3. Record your data. 4. Pour HCl or vinegar into two small cups. Place the one larger "cell" into one cup and the two smaller cells in the other cup. Start timing 30 minutes. 5. After 30 minutes, remove the cells and blot them dry with a paper towel. 6. Using your ruler, measure the distance the HCl has diffused into the blocks as shown on the…TOPIC: KATO-THICK & KATO-KATZ TECHNIQUES 1. List down 2 advantages and disadvantages of Kato-Thick method? Briefly explain each.
- Activity 2. Media Used in Isolating Coliforms You are a group of microbiologists tasked to test the presence of coliforms in the newly built water station. You are asked to isolate E. coli, Salmonella, and Shigella. Enumerate the media used for each step of isolating these bacteria. Include pictures and references. Bacteria Enrichment (Broth/Agar) Presumptive test (Broth/Agar) Isolation Media (Broth/Agar) E. coli Salmonella Shigella 1. How can you confirm that E. coli, Salmonella, and Shigella are present in the newly built water station? Explain. 2. What is the difference between nutrient broth and nutrient agar? 3. What is the importance of these steps (Enrichment, Isolation, and presumptive test) in isolating bacteria? Conclusion about the process of isolating bacteriaQuestions: 1. What happens to the number of bacteria as you streak from one sector to another? 2. What happens to the colonies when the bacteria are separated well? 3. How bacteria does each colony come from? many 4. Why do you make sure that the inoculating loop is red hot? 5. What happens when the streaking is not correct? 6. When do you flame the mouth of the cultures tubes? 7. Why you should not use the inoculating loop when it is red hot?webapps/assessment/take/launch.jsp?course_assessment_id%3D 498749 1&course_id3 111786 1&content id=D Customize Links Free Hotmail Windows Windows Media Imported From IE Importe Serial dilution QUESTION 2 What type of agar is TSA? (select all that apply) Nutrient