Comparing the active site geometries of chymotrypsin and subtilisin enzymes under the hypothesis that their similarities had catalytic importance led to a greater mechanistic knowledge of both enzymes (which they did not). Can you discuss the efficacy of this tactic?
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Comparing the active site geometries of chymotrypsin and subtilisin enzymes under the hypothesis that their similarities had catalytic importance led to a greater mechanistic knowledge of both enzymes (which they did not). Can you discuss the efficacy of this tactic?
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- Comparing chymotrypsin and subtilisin active site geometries on the assumption that their similarities have catalytic importance has improved mechanistic knowledge of both enzymes (which they did not). Can you evaluate this approach?The comparison of the active site geometries of chymotrypsin and subtilisin under the assumption that their similarities have catalytic signifi cance has led to greater mechanistic understanding of both these enzymes. Discuss the validity of this strategy.Increased mechanistic knowledge of both chymotrypsin and subtilisin enzymes has emerged from comparing their active site geometries in the hope that their commonalities would have catalytic importance (which they did not). Are you able to discuss the efficacy of this strategy?
- The comparison of chymotrypsin and subtilisin active site geometries on the assumption that their similarities had catalytic importance has improved mechanistic knowledge of both enzymes (which they did not). Is this technique effective?The Commercial Trainee Program of the Pharma Biotec Industry, opened selection to admit candidates who would help in the development of a more efficient production protocol of Ibuprofen, a non-colloidal anti-inflammatory. Basically, the challenge stimulated obtaining high enantiomeric purity of the final product. In this way, the candidates proposed the use of immobilized enzymes for the process. Tick the most coherent alternative to justify the choice of using immobilized enzymes by the candidates for the case mentioned above in the question. (Only one statement is true)* A)The use of immobilized enzymes was an interesting strategy, since different immobilization techniques can be used, such as adsorption and covalent. b) The use of immobilized enzymes was consistent since the immobilization process can further favor the regioselectivity , enantioselectivity and stereoselectivity properties of the enzymes, which will favor the enantiomeric purity of the drug. c) The use of…Mechanistic insights into the catalytic mechanisms of chymotrypsin and subtilisin were gained by a comparison of their active site geometries (which they did not). What do you think of the effectiveness of this strategy?
- Briefly describe why the Michaelis-Menten equilibrium-assumption cannotbe applicable to all enzyme-catalyzed reactionsPut these steps in the mechanism of chymotrypsin catalysis in order from first to last. Note that some references may not break out each of these steps individually, but all steps should be ordered. There will be eight steps.how allosteric regulation is fundamentally different from competitive/uncompetitive/mixed inhibition and why Michaelis-Menten and Lineweaver-Burk analysis cannot be used for allosteric enzymes.
- Enzymes are stereochemically specific; that is, they oftenconvert only one stereoisomeric form of substrate intoproduct. Why is such specificity inherent in theirstructure?Only a few amino acid residues are actually involved in catalysis in enzymes, yet enzymes are constructed of at least 100 amino acids, and often many more. Suggest some functions for the noncatalytic amino acids.Enzymes act by reducing the activation energy of areaction. Describe several ways in which this isaccomplished.