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Besides your mycobacteria, what other microorganism can appear as acid fast positive when processed using acid fast staining?
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- Which of the following is/are true regarding the acid-fast stain? (There may be more than one correct answer.) Non-acid-fast microbes appear blue in a completed acid-fast stain. O Acid-fast cells retain the primary dye after treatment with acid-alcohol. O It is used to identify members of the genus Mycobacterium. V Acid-fast cells appear bright pink/red in a completed acid-fast stain. O If cells are acid-fast, they are gram-negative.The acid-fast bacterium, Mycobacterium smegmatis, is relatively safe to be used by students in performing the acid-fast stain technique because of its thin cell wall. However, if this organism is used for Gram-stain, it can also take the Gram stain reaction. Why is this so?Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?
- If cultures are suffering severely due to alkaloid production and accumulation in the medium then, how can i overcome this problem?What advantage(s) does nucleic acid amplification have overstandard culture methods for identification of microorganisms?What are the disadvantages?What is the purpose of acid-fast staining? What information can you gain from this procedure? Please explain.
- Why is it essential that smears be air-dried? Why can’t they be gently heated over a flame to speed up the drying process?Acid-Fast stain A) E. Coli -Morphology shape -Arragement -Acid-fast or non-Acid-fast B) S. Epidermidis -Morphology shape -Arragement -Acid-fast or non-Acid-fast(a) Which would be easier to prepare bacterial smear from, liquid or solid culture? Why? (b) Aside from bacteria, can gram staining be used with microbes such as fungi? Explain your answer.
- In preparing a bacterial smear for staining, heat fixation is done after the smear dries up.a.) Give the purpose of heat fixation.b.) What can be observed in wet mounts or hanging-drop slides that cannot be observed in heat-fixed slides?Your instructor asks you to isolate and identify the organisms in an unknown culture. You find that the culture contains two gramnegative bacilli that produce swarming colonies. What biochemical test would you use to identify the bacilli? Justify your answer.Explain why only gram-negative cells undergo decolorization during the gram staining procedure. Cite the purpose of each of the following reagents in a differential staining procedure. Primary stain Mordant Decolorizing Agent Counter stain What might happen if the Gram staining procedure is performed on a culture incubated for a little over a day