Below are photos of 3 bacteria, letter lableled, inoculated onto MSA plates and incubated. Note no growth on plate C B MSA (Mannitol Salt Agar)
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- If some cells in your Gram stain are red/pink rods and some are purple cocci, __________. your smear was not made from a pure culture your unknown bacterium is a pleomorphic species the bacteria ferment sucrose your unknown is a species of Escherichia you should inoculate an EMB agar plateThe figure attached shows the colonies on Eosin Methylene Blue Agar (EMBA) plate. Make a description on the picture.Microorganism Type of Microorganism Gram Stain Shape Tryptic Soy Agar McConkey Agar Blood Agar EMB Agar Mannitol Salt Agar Bacillus cereus Bacillus megaterium Bacillus subtilis Candida albicans Escherichia coli Micrococcus luteus Neisseria sicca Proteus vulgaris Pseudomonas aeruginosa Serratia marcescens Staphylococcus aureus Staphylococcus epidermidis
- Special Media for Isolating Bacteria OBJECTIVES Because multiple methods and multiple media exist, you must be able to match the correct procedure to the desired microbe. For example, if bacterium B is salt-tolerant, a high concentration (>5%) of salt could be added to the culture medium. Physical conditions can also be used to select for a bacterium. If bacteri- After completing this exercise, you should be able to: 1. Differentiate selective from differential media. 2. Provide an application for enrichment and selec- tive media. um B is heat-resistant, the specimen could be heated before isolation. Dyes such as phenol red, eosin, or methylene blue are sometimes ineluded in differential BACKGROUND media. Products of bacterial metabolism can react with One of the major limitations of dilution techniques used to isolate bacteria is that organisms present in limited amounts may be diluted out on plates filled with dominant bacteria. For example, if the culture to be isolated has 1…Gas Gangrene: You are working in the Surgery Unit and are helping a paitent with "gas gangrene". Samples of tissues and fluid delivered to lab for culture and identification. Microbes could not grow aerobically; growth occurred anaerobically only Blood agar after anaerobic incubation (below), note zones of clearing around colonnies:S aureus Styphimurium S aureus S typhimurium Nutrient Agar Plates MacConkey Agar Plates S epidermidis Unknown S epidermidis Unknown S aureus S typhimurium S aureus S typhimurium Mannitol Salt Agar Plates Gram Stain S epidermidis Unknown S epidermidis Unknown
- Which of the following tests will help me differentiate between an unknown bacteria that is either Salmonella typhimurium and Serratia marcescens Wet Mount (motility testing) Endospore Staining Acid Fast Staining Differential & Selective Media (MacConkey, PEA, EMB, MSA Agar, Blood Agar, etc.) Oxidative-Fermentative Test Carbohydrate Fermentation MR-VP Testing Catalase TestDescribe characteristics of Streptococcus Agalactiae in the Agar: (How does colonies look like (color) and explain does it grow on that agar. (Don't have to write the incubation period) ~ Only describe how would it look like on the Agar: Blood Agar (Aerobic) MacConkey EMB PEA Mannitol Salt Agar Chocolate Agar Nutrient AgarThe following is a picture of Eosin Methylene Blue Agar. What does the growth on this plate indicate? ASM MicrobeLibrary.org © Cheeptham CAt the Brewery part 2: Continue identifying microbes you islated from the contaminated beer: Microbe 2: top photo growth on Sabauraud Dextrose agar; bottom microscopic viewMSA (Mannitol Salt Agar) Match the description to the correct Bacterium A= growth, agar is yellow B= Growth, agar is not yellow C= no growthDescribe characteristics of Streptococcus Agalactiae in the Agar: (How does colonies look like (color) and explain does it grow on that agar. Blood Agar (Aerobic) MacConkey EMB PEA Mannitol Salt Agar Chocolate Agar Nutient AgarSEE MORE QUESTIONS