a. Use circles for activators and squares for repressors. Draw the shapes bound to each promoter region. b. Under the word gene write “ON” or “OFF” to indicate if the gene is transcribed or not under the different conditions.
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a. Use circles for activators and squares for repressors. Draw the shapes bound to each promoter region.
b. Under the word gene write “ON” or “OFF” to indicate if the gene is transcribed or not under the different conditions.
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- 4. Which or which (can select more than one alternative) of the control methods of genetic expression in eukaryotes NOT USED in prokaryotic cells?to. control how often a gene is expressedb. control how an mRNA is processedc. control which mRNA is exported from the nucleus to the cytosold. control which mRNA is translated to proteins in the ribosomeand. control how quickly proteins are destroyed once synthesized 5. Unlike DNA that usually forms a helical structure, different RNA molecules assume bends in a wide variety of 3D shapes. This is due to :to. RNA contains uracil and ribose as sugarb. the bases in the RNA cannot form hydrogen bonds with each otherc. nucleotides in RNA have a different chemical structure than they have in DNAd. Although RNA is single-stranded, complementary bases can pair with each other. 6. Viruses consist of a layer of protein that surrounds your genetic material. the genetic material in the HIV virus is:to. Double-stranded DNA (dsDNA)b. Single-stranded DNA…Which of the following describes transcription factors? The addition or deletion of methyl and acetyl groups to regulate transcription Proteins that specifically bind to promoter or enhancer regions to initiate transcription The transcription initiation sites of DNA that bind RNA polymerase DNA regulatory elements that actively repress the transcription of a gene O Varying sequences that are or are not included in the instructions to make a protein2. You are excited because you discovered a DNA sequence motif that appears to be in the promoters of very highly expressed genes. You suspect that it is the binding site for a transcription factor (TF). The problem is, that you can't identify the TF! The motif you found is: A-A-T-C-G-C/G-A/T-A/T-C/G-A/T where 'X' is any nucleotide and '/' denotes either of the two nucleotides. a) You give up trying to identify the TF and decide that you are going to engineer your own TF. How might you go about synthetically creating a novel TF that best targets this sequence? What considerations would you need to take into account here? b) How might you go about confirming that this sequence is indeed important for gene expression?
- 3. Differentiate between factors and processes involved in transcription: Recognition of this DNA sequence is [Choose] required for transcription to initiate:. [ Choose ] PROMOTER Occurs when the ribosome reaches a RNA POLYMERASE stop codon on the MRNA TERMINATION INITIATION SITE ENHANCERS The main enzyme responsible for transcribing DNA into RNA is ELONGATION Will increase the efficiency of gene [Choose] expression, but are not essential for transcription. RNA polymerase "walks" along the template strand, adding a [Choose ] matching (complementary) RNA nucleotide to each existing ncleotide. The section of DNA from which the [Choose ) very first 5" RNA nucleotide is transcribed Question 4 1 pts hp 近Choose all that apply regarding gene transcription in eukaryotes: Exons are removed from mRNA by the spliceosome. RNA polymerase II must completely finish an mRNA transcript before processing can begin. RNA polymerase I catalyzes the synthesis of the majority of ribosomal RNA. Histone acetylation is a method of controlling gene expression. Acetylation creates more positive charges on histones, leading to tighter binding of the proteins to DNA. Introns must be removed from the initial RNA transcript. The hormone 17beta-estradiol binds to a G-protein coupled receptor to control gene transcription. The 5' cap of mRNA requires the free triphosphate on the nucleotide at the 5' end. Multiple transcription factors are necessary to form the pre-initiation complex (PIC) of RNA Pol II.1. What happens during transcription? Possible sentence frame: Transcription is the process in which the information from converted into This happens by is
- How can transcription factors influence the transcription of DNA? Select all that apply. Inhibit transcription by preventing RNA polymerase from binding to promoters Inhibit translation by preventing the binding of mRNA to ribosomes Enhance transcription by helping RNA polymerase bind to promoters Inhibit transcription by blocking the progress of RNA polymerase along a DNA molecule1. DNA is a linear code of biological information in the form of genes. DNA is transcribed to another linear molecule RNA, which is translated into a linear polypeptide. For each gene, there sites which control the transcription and translation and sites which defines the beginning and end of the gene, the mRNA and the polypeptide. For a single gene, its mRNA and the resulting polypeptide, draw all the three molecules in parallel and indicate the control sites and those which mark the beginning and end of each.1. (a) Binding of transcription activator protein Gene is switched ON Gene is switched OFF Does NOT affect the gene expression (b) the RNA polymerase binds at the promoter region Gene is switched ON Gene is switched OFF Does NOT affect the gene expression
- 8. Label each figure with what TYPE OF CELL is undergoing gene expression: Nuclear membrane DNA Cytoplasm Plasma membrane Nucleus Cytoplasm Mature mRNA Pre-MRNA MRNA DNA Protein Ribosomes Protein RibosomesWhich of the following functions are characteristic of general transcription factors in eukaryotes? Hint: there may be more than one correct response. Be sure to check all that apply. They inhibit RNA polymerase binding to the promoter and begin transcribing. They bind to other proteins or to the TATA box. They are sufficient to allow high levels of transcription. O They initiate and regulate the transcription of genes. O They bind to sequences just after the start site of transcription.Which of the following is an example of positive control? (a) transcription occurs when a repressor binds to an inducer (b) transcription cannot occur when a repressor binds to a corepressor (c) transcription is stimulated when an activator protein binds to DNA (d) a and b (e) a and c