A test procedure calls for 5 mL of undiluted specimen. 2 mL of a 1:50 dilution is used. The answer obtained is 30 mg/dL. What answer should be reported?
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A test procedure calls for 5 mL of undiluted specimen. 2 mL of a 1:50 dilution is used. The answer obtained is 30 mg/dL. What answer should be reported?
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- A plate has 72 colonies with a total dilution factor of 10-7, 0.1 ml was pipetted onto the plate, what was the original CFU/ml concentration of that sample?Prepare 50 plates of Sheep's Blood Agar using 100mm petri dish. Add 10% of blood to the media. (Manufacturer's instructions: Suspend 38 g of blood agar base dehydrated powder in 1 liter of distilled or deionized water.) Pour 25 mL of prepared medium per plate. Express answer to the nearest whole number. How many grams of the dehydrated powder should be weighed? Answer: What is the volume of distilled water that should be used in the preparation? Answer: What is the volume of sheep's blood that should be added to the sterilized and cooled blood agar base? Answer:A bacterial suspension was diluted and 0.1 mL of each of the dilutions was plated in duplicate. The following results were obtained. What was the original concentration? Dilution CFU Counted CFU Counted 10-2 29 24 10-3 1 3 10-4 0 0
- Based on the following data, what is the MRSA concentration (CFU/ml) in the patient's blood sample? Write your answer in the following format: Value x 10^Exp. Do not include units. 100 μl 100 μl 100 μl 2.8X10^2 A Original 100 μl 100 μl Blood Sample 100 μl B 900 μl c 900 μl D 900 μl E 900 μl F 900 μl Saline Tubes Lawn of growth 000 100 μl 200080 100 μl 100 μl | 100 μ. | 100 με 280 22 2 0 colonies colonies colonies colonies LB + MethicillinThe table below summarizes the results for Lead-sulfide test. Provide the correct remarks from the results if Negative for Lead-sulfide test or Positive for the Lead sulfide test and it's either Negative for -SH or Positive for -SHTo the right is an image of a dilution that was performed. The volume above the top arrow indicates the volume that should be transferred to the next tube (i.e. Tube 1). The volume listed at the bottom-right of tube 1 indicates the volume of diluent that should be added to that tube. The stock concentration is 50μg/ml and you want to make a solution in tube 1 with a concentration of 0.4μg/ml and a total volume of 3ml. Stock ?ml Tube 1 ?ml a. How many milliliters of stock solution needs to be added to Tube 1? Round your answer to three decimal places (e.g. 0.111). b. How many milliliters of diluent needs to be added to Tube 1? Round your answer to three decimal places.
- An original sample of water containing 4.00 X 106 CFU/mL was diluted by 4 successive 1/10 dilutions. After incubation, 200 colonies were found growing on the plate. How many mLs from the last dilution tube were plated out?Define D-dimer test and how to interpret the test. The reference range is in the table below Test Result (reference range) D-dimer, ng/mL FEU > 4000 (< 500)What is the purpose of the RPR (RAPID PLASMA REAGIN ) Test? Why is a qualitative test performed before a semi-quantitative test? Why is it necessary to rotate the slide/card?
- 6) 1 mL of supernatant is required for a procedure. The final colored solution proves to be too high to read accurately on the spectrophotometer. 100 μL of supernatant and 900 μL of distilled water are substituted for the original supernatant and the procedure, run as before. The reading from the standard curve is 46 mg/dL. What is the actual amount of substance in the patient serum?To determine the cfu/ml of a pondwater sample, you perform 1/10, 1/100, 1/1000 and 1/10,000 serial dilutions and plate 0.1 mls of each dilution. You count 40 colonies on the plate derived from the 1/10,000 dilution. What is the cfu/ml of the pondwater? Show your work and use scientific notation.The following are errors that people commonly make when they perform serial dilutions. Indicate whether you think that the number of cfu/ ml calculated would be too high or too low if you make this mistake. You intend to add 0.9 ml of diluent to each tube and 0.1 ml of culture. Instead, you add 0.5 ml of diluent to each tube and 0.1 ml of culture to the first tube. Then, you make a serial dilution of 0.1 ml into and from each tube as described. You prepare 0.9 ml of diluents in each tube. You add 0.1 ml of culture (from the overnight culture provided) to every tube. You add 0.9 ml of diluent to each tube. You add 0.1 ml of culture to the first tube and mix. You get distracted, and transfer 0.1 ml to the third tube instead of the second. You perform the rest of the series as described.
